The Iwahori subgroup $I \subseteq \mathrm{GL}_n(k)$: matrices whose diagonal entries are units of $\mathcal O$, strictly-above-diagonal entries lie in $\mathcal O$, and strictly-below-diagonal entries lie in the maximal ideal $\mathfrak m$.
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The upper unipotent subgroup $N \subseteq \mathrm{GL}_n(k)$: upper unitriangular matrices, i.e. matrices with $1$s on the diagonal and $0$s strictly below it.
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The lower unipotent subgroup $N^{\mathrm{op}} \subseteq \mathrm{GL}_n(k)$: lower unitriangular matrices, i.e. matrices with $1$s on the diagonal and $0$s strictly above it.
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The diagonal subgroup $M \subseteq \mathrm{GL}_n(k)$: matrices that vanish off the diagonal.